The following procedure and protocol manuals are provided for our microbiology laboratory and are available as an educational reference for other laboratories all manuals are in pdf format to maintain internal links we recommend the use of microsoft internet explorer. Microbiology laboratory manual 17 naveena varghese joy p p 20 14 pineapple research station k erala agricultural university vazhakulam 686 670 muvattupuzha ernakulam kerala. The manual covers lab safety how to use a microscope aseptic transfer of bacteria simple gram capsule acid fast spore and negative staining environmental requirements of bacteria antibiotic susceptibility testing bacterial control methods membrane filtration standard plate count plaque assay biochemical tests for the identification of pathogenic enterobacteriaceae an unknown lab staphylococcus and streptococcus bacteria and elisas. This microbiology laboratory manual is designed especially for the non majors health science microbiology courses the organization reflects the body systems approach and contains specific sections on clinical diagnosis 36 exercises and 43 experiments cover a broad range of topics features o an emphasis is placed on the basic principles of diagnostic microbiology and the lab procedures